filiformis, S. moorei and E. rhusiopathiae (926, 1,124, 1,035 and 994, respectively). However, the distribution of genes into COG categories was almost similar in all compared genomes (Figure 7). Figure 7 Distribution of functional classes of predicted genes on Holdemania massiliensis (colored in blue), Holdemania filiformis (colored in red), Solobacterium moorei (colored in green) www.selleckchem.com/products/Bortezomib.html and Erysipelothrix rhusiopathiae (colored in yellow) chromosomes according … The nucleotide sequence identity ranged from 62.02 to 84.32% among compared genomes. Table 6 summarizes the numbers of orthologous genes and the average percentage of nucleotide sequence identity between the different genomes studied.
Table 6 The numbers of orthologous proteins shared between genomes (upper right)* Conclusion On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Holdemania massiliensis sp. nov. that contains the strain AP2T. This bacterial strain has been found in Marseille, France. Description of Holdemania massiliensis sp. nov. Holdemania massiliensis (mas.si.li.en��sis. L. masc. adj. massiliensis of Massilia, the Roman name of Marseille, France, where the type strain was isolated). Colonies were 0.2 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion agar. Cells are rod-shaped with a mean diameter of 0.57 ��m and a mean length of 1.75 ��m. Strictly anaerobic. Growth occurs between 25 and 45��C, with optimal growth observed at 37��C. Cells stain Gram-positive, and are non-motile.
Cells are positive for ��-galactosidase, ��-glucosidase, ��-fucosidase and pyroglutamic acid arylamidase. Positive reactions were observed for glycerol, D-ribose, D-galactose, D-glucose, D-fructose, D-mannose, inositol, D-mannitol, D-sorbitol, N-acetyl-glucosamine, amygdalin, arbutin, esculin, salicin, D-cellobiose, D-maltose, D-lactose, D-saccharose, D-melezitose, gentiobiose, D-tagatose and potassium gluconate. Cells are susceptible to amoxicillin, metronidazole, vancomycin, clindamycin and imipenem. The G+C content of the genome is 47.10%. The 16S rRNA and genome sequences are deposited in GenBank under accession numbers “type”:”entrez-nucleotide”,”attrs”:”text”:”JX101683″,”term_id”:”399893006″,”term_text”:”JX101683″JX101683 and “type”:”entrez-nucleotide”,”attrs”:”text”:”CALK00000000″,”term_id”:”399173253″,”term_text”:”CALK00000000″CALK00000000, respectively.
The type strain AP2T (= CSUR P195 = DSM 26143) was isolated from the fecal flora of French Caucasian female suffering from severe restrictive anorexia nervosa . Acknowledgements The authors thank the Xegen Company (www.xegen.fr) for automating the genomic annotation process. This study was funded by the Mediterranee-Infection Foundation.
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