This lignocellulolytic residue has been used as substrate in mush

This lignocellulolytic residue has been used as substrate in mushroom production (Dias et al., 2003). It is important to point out that the use of these residues in the production of mushrooms prevents their direct release into the environment, increases the producer’s

income and leads to food product with high nutritional quality. Mushroom yields and their chemical composition can be affected by the substrates used in their growth (Shashirekha, Rajarathnam, & Bano, 2005). For instance, yields and chemical composition are enhanced by adding essential elements, such as Se, to the substrate. Addition of sodium selenite to the substrate used for growing Ganoderma Navitoclax cell line lucidum resulted in a proportional increase of Se content in the mushrooms ( Zhao et al., 2004). Studies have revealed that Se is incorporated into the P. ostreatus biomass, as this element was found to be associated with the membrane (44%) and cell wall (56%). Se incorporation into fungal proteins reveals a great potential to improve the nutritional value of the mushroom ( Munoz et al., 2006). In enriched mushrooms, the Se bioavailability was verified using in vivo methods. The higher

levels of absorption of Se in rats fed with Se enriched mushrooms were verified by Silva et al. (2010), which compared these results with the ones achieved Veliparib ic50 with rats with sodium selenate in their diets. Due to the high demand of food across the world, its enrichment with essential micronutrients, such as Se, is crucial. MycoClean Mycoplasma Removal Kit However, Se

can also be toxic when ingested in high concentrations (Gaso et al., 2000 and Hartikainen, 2005). The recommended dose for an adult, male or female, is 55 μg day1 (IOM, 2000). Selenium has several physiological functions in protein activity, enhancing immune system function, reducing cancer risk (Finley, 2006), collateral effects of chemotherapy (Sieja & Talercszyk, 2004) and functional activity of cancer metastasis (Finley, Sigrid-Keck, Robbins, & Hintze, 2005). Thus, the aim of this work was to evaluate the use of coffee husk in the production of Pleurotus ostreatus mushrooms enriched with selenium. The fungus used was P. ostreatus, and inoculation was performed in rice cooked with water for 50 min and autoclaved at 121 °C for 2 h. The coffee husk substrate was obtained from the Incofex Coffee Corporation, in Viçosa, Minas Gerais State, Brazil. The husk was boiled in water for 2 h, in order to reduce some compounds which could inhibit fungal growth and contaminants, and centrifuged at 1800 rpm for 5 min to remove excess water. Next, 1.5 kg of each sample were placed in polypropylene bags and autoclaved for 2 h. This procedure was repeated three times at 48-h intervals. The final humidity was 80%.

This study has highlighted phytochemical accumulation for rocket

This study has highlighted phytochemical accumulation for rocket varieties and accessions grown under controlled conditions. This is in contrast to field conditions that often stress plants and create phytochemical profiles reflective of fluctuating environmental stresses such as light intensity, temperature, pests and diseases. These studies, whilst undoubtedly valuable to rocket salad research, are not always directly comparable with other growing regions

and climatic backgrounds. It has been demonstrated in this study that under controlled conditions, and therefore due to genetic regulation rather than environmental response, that rocket predominantly accumulates glucosativin, and that virtually all other glucosinolates detected were GDC0449 minor by comparison. There was significant variability

in these accumulations between varieties, providing scope for plant breeders to select varieties based on their baseline accumulations of health-beneficial precursors such as glucoraphanin and glucoerucin. This can also be said of flavonol compounds detected this website in rocket. Significant variability was detected between accessions, and high accumulators may be a valuable genetic resource for breeders. By determining the baseline accumulations of phytochemicals in this manner, varieties can then be tested in a field environment to ascertain any differences that could affect commercial production. Several previous studies have made mention of using phytochemical screening as a means of selecting accessions to introduce into breeding programs. In almost every instance however, the experimental design of these studies was flawed by the fact that time-of-harvest was either much too early or much too late relative to Selleck Gemcitabine the commercial average. Not only does this make comparing results between studies more difficult, it also ignores the fact that phytochemical concentration and profiles

change as plants grow (Fernandes, de Pinho, Valentao, Pereira, & Andrade, 2009). If researchers wish to make their data as useful to breeding programs as possible, the phytochemical profile must be determined at the point of commercial harvest, as this is when concentrations will be at their most useful in a “real-world” commercial setting. Plant breeders and food processors will not be interested in the phytochemical content of seedlings or of plants that have bolted or flowered (unless they provide products for a very niche market), as their customers will not eat the product at these points. Table 3 features the number of days each of the mentioned studies grew rocket plants before harvesting. Regardless of growing conditions, the number generally chosen seems arbitrary.

In general, the implant procedure was safe There were no operati

In general, the implant procedure was safe. There were no operative deaths (i.e., there was no mortality within 30 days of the implant procedure). Three deaths occurred between 31 days and 6 months follow-up, including 1 adjudicated as device-related. This last patient developed a procedure-related sternal wound infection post-operatively with presence of Methicillin resistant Staphyllococcus aureus (MRSA) in cultures. The infection remained unresolved over several months despite repeated antibiotic treatments and debridement. After a CT identified a fistula track from the sternum to the device, the patient was taken to the operating

room for sternectomy and pectoral flap reconstruction. During resternotomy, atrial and aortic tears occurred, and the patient died intraoperatively. One patient died 60 days after implant buy ISRIB and another at 61 days after implant; both deaths were adjudicated as non–device related by the independent Clinical Events Committee. One patient underwent LVAD implant, another underwent heart transplant. One patient had the PIL and cuff removed at the 6-month visit because of a disrupted internal gas-line following a fall that damaged the line. Between 6 and 12 months, 1 patient had a heart transplant, 1 received an LVAD, 1 was weaned from therapy at 11 months for left ventricular RNA Synthesis inhibitor recovery, and 1 discontinued therapy voluntarily and had

the PIL explanted selleck chemicals llc following the 6 months post-implant follow-up visit. Some

patients included in the study were in late-stage heart failure disease. While it was our intent to treat patients who were not candidates for LVAD or transplant, some of these patients were evaluated for transplant at baseline. Two patients continued having supraventricular arrhythmia despite cardioversion and/or ablation and went to transplant. One patient had opted out of LVAD, but repeat arrhythmias led to a LVAD implant. One patient was scheduled to have a PIL replacement when the surgeon made the decision to implant a LVAD instead. One-year survival was 85%. Table 3 presents the primary safety endpoint analysis at 6 and 12 months. The composite device-related adverse event rate through 6 months, as classified by the Clinical Events Committee, was 50%. This result was influenced by the exit site infection rate of 40%. Between 6 months and 12 months, there were no additional patients with device-related serious adverse events. Table 4 presents the efficacy analysis at 6 and 12 months. Significant improvements were noted in NYHA functional class at both 6 and 12 months. Four (20%) and 3 (15%) patients were asymptomatic at 6 and 12 months, respectively, improving from NYHA functional class IV or III to functional class I (Figure 2). The Minnesota Living with Heart Failure QoL score significantly improved at 6 and 12 months. The Kansas City Cardiomyopathy Questionnaire score also significantly improved at 6 and 12 months.

By contrast, if no sugar moieties were attached to the 20th carbo

By contrast, if no sugar moieties were attached to the 20th carbon of the ginsenosides such as Rg3, Rh2, and Rg2, the ginsenosides acted as a prooxidant. In ginsenosides such as Rh1, glucose is attached to the sixth carbon instead of the 20th, and in this case, the ginsenoside acts as an antioxidant only [29]. All these aggregated reports revealed that the prevention of ROS generation

by ginseng may be an important milestone in the prevention of oxidative damage. Ginsenoside Rb1 has protective effects on human umbilical vein endothelial cells in vitro [30]. Water extract of Korean red ginseng stimulates angiogenesis by activating the phosphoinositol-3-kinase GSK126 (PI3K)/Akt-dependent extracellular signal-regulated kinase 1/2 and endothelial nitric oxide synthase (eNOS) pathways in human umbilical vein endothelial cells [31]. Angiomodulatory and neurological effects are also shown by ginsenosides [32]. One study shows that potassium channels of vascular smooth muscle PD-1/PD-L1 mutation cells have

been activated by ginsensoside Re through the PI3K/Akt and NO pathways [33]. Another study shows that ginsenoside Re has nongenomic effects in endothelial cells through the glucocorticoid receptor (GR) [34]. Capillary morphogenesis was attenuated by ginsenoside Rb1 [35]. Another in vitro study revealed the enhancement of vascular endothelial cell proliferation and migration by extracts of P. ginseng and P. notoginseng [36]. Saponin from P. notoginseng shows angiogenic effects on both human umbilical vein endothelial cells and in zebrafish models [37]. It is also reported that atherosclerotic lesions in apolipoprotein E (ApoE)-deficient

mice and tumor necrosis factor-alpha-induced endothelial adhesion molecule expression have been reduced by P. notoginseng [38]. Production of NO was increased Pyruvate dehydrogenase lipoamide kinase isozyme 1 by ginsenoside Rg3 by increasing phosphorylation and expression of eNOS [39]. In human umbilical vein endothelial cells, fibroblast growth factor-induced angiogenesis was inhibited by compound K through the modulation of p38 mitogen-activated protein kinase (PK) and Akt [40]. The aforementioned reports propose that the saponin extracted from ginseng protects vascular endothelial cells through the NO-, Akt-, and GR-mediated signaling pathways. Effects of ginseng and ginsenosides have been sufficiently studied on the cardiovascular system. Through the production and release of NO, endothelium regulates blood vessel tone [41], [42] and [43]. Production of NO has been stimulated by ginsenosides by a number of ways. It is reported that NO production in human aortic endothelial cells was induced by purified ginsenoside Rb1 [44]. Ginsenoside stimulates NO release in human umbilical vein endothelial cells by phosphorylation of GR, PI3K, Akt/PKB, and eNOS [45]. In isolated canine corpus cavernosum model, ginsenoside Rg3 induced vasodilation [46], which shows that arterial stiffness has been improved by Korean red ginseng and ginsenosides [47].

To detect the duration and frequency of WSB outbreaks dendrochron

To detect the duration and frequency of WSB outbreaks dendrochronological studies commonly remove the climate-driven component of radial growth contributing to inter-annual variation. This variation is ‘corrected’ using a chronology from a non-host tree species, i.e., a tree species that is not defoliated by the budworm, but is sensitive to the same climatic conditions as the host. Periods of sustained growth reduction remaining in the corrected host chronology are inferred to result from http://www.selleckchem.com/EGFR(HER).html WSB defoliation (Swetnam and Lynch, 1989). The Cariboo Forest Region extends from 51°00′ to 52°30′ north latitude and from 120°30′ to 125°45′

west longitude in the BC central interior (Fig. 1). The Fraser Plateau makes

up a large portion of the region and is characterized by a level to gently rolling landscape incised by river valleys, and local uplands with elevations predominantly ranging from 900 to 1500 metres above sea level (masl). The Chilcotin Plateau extends along the western periphery of the region, beyond which the Coast Mountains rise sharply to elevations up to 4000 masl. This landscape configuration results in a strong rain shadow effect and the western Chilcotin is the driest CAL-101 chemical structure portion of the study area, with average annual precipitation at Tatla Lake averaging 403 mm/yr. As Pacific air masses move further eastward towards Williams Lake, humidity levels and precipitation increase slightly, with annual precipitation totals averaging 417 mm/yr Bay 11-7085 (Wang et al., 2012). Summer months are typically dry, with most precipitation resulting from numerous convective storms. In the winter months Arctic air masses result in extended periods of extreme cold temperatures (Steen and Coupé, 1997). In BC the biogeoclimatic ecosystem classification

(BEC) uses vegetation, soils, and topography to identify geographic areas, referred to as biogeoclimatic zones, which have a relatively uniform climate. BEC zones are further divided into subzones based on the moisture and temperature regime of the area, respectively and some BEC subzones are further classified into variants based on their location or distribution within a subzone (Meidinger and Pojar, 1991). In the Cariboo Forest Region, the Interior Douglas-fir (IDF) BEC zone makes up approximately 45% (17,000 km2) of the area and is located above the valleys of the Fraser, Chilcotin, and Chilanko rivers (Steen and Coupé, 1997). The very dry-warm (xw) and very dry-mild (xm) subzones are the driest and warmest in the region, and are transitional between grassland and forest (Table 2). The dry-cool (dk) subzone covers the largest area in the Cariboo Forest Region and is comprised of four variants, with the Chilcotin variant (dk4) being the coldest and driest (Steen and Coupé, 1997; Table 2). Herein, we shall refer to BEC subzones (e.g., xm) and BEC variants (e.g., dk4) simply as BEC units.

The distinction between below- and aboveground biomass was based

The distinction between below- and aboveground biomass was based on the arbitrary position of the ground surface. In some ecosystems, a considerable proportion of the roots occur above the ground surface and likewise, part of the stem biomass sometimes occurs below the soil surface (Mokany et al., 2006). There might be some disagreement about considering the 15 cm of Stu aboveground

as a belowground component, but the Stu only represented Epigenetics inhibitor 5–6% of the total tree biomass. The root:shoot ratio does not represent the total C allocation to the tree compartments, since it does not incorporate the considerable loss of C resulting from respiration and senescence (turnover). So, the root:shoot ratio only represents the net effects of carbon allocation. Although root:shoot ratios may only be rough indicators of physiological processes affecting C allocation, they are

very valuable for providing estimates of belowground plant biomass from aboveground biomass. For example, multiplying the biomass of the tree organs by its turnover and decomposition rates indicates that C allocation in trees strongly influences forest C cycling. Consequently a proper understanding of C allocation is an important issue in the context of best management practices for biomass production and C sequestration in the soil. The coppice of the aboveground biomass resulted in a large Fr mortality

and a tremendous input of C to the soil. The results obtained after coppice Tyrosine Kinase Inhibitor Library mouse could be confounded with the tree ontogeny, and a control without coppicing at year 3 and 4 would have been useful. However, that experimental design was not possible as the plantation was treated as a commercial plantation with homogenous management in the whole area. Carbohydrate Larger trees stored significant amount of C belowground with bigger root system, but bigger trees did not necessarily produce more fine roots. Both poplar genotypes only rooted in the upper 30 cm, and they showed relatively shallow, but widespread root systems. These results have implications for the design of C sequestration strategies. This work was supported by the European Research Council under the European Commission’s Seventh Framework Programme (FP7/2007-2013) as ERC Advanced Grant agreement # 233366 (POPFULL), as well as by the Flemish Hercules Foundation as Infrastructure contract ZW09-06. Further funding was provided by the Flemish Methusalem Programme and by the Research Council of the University of Antwerp. GB was supported by the Erasmus-Mundus External Cooperation, Consortium EADIC – Window Lot 16 financed by the European Union Mobility Programme # 2009-1655/001-001.

6) In Fig 6 (E-SAL), epithelial and endothelial cells were not

6). In Fig. 6 (E-SAL), epithelial and endothelial cells were not present. BMDMC attenuated all these ultrastructural changes and yielded multinucleated cells and type II pneumocytes with large hyperplasia of lamellar bodies. Additionally, epithelial and endothelial cells were noted around the interstitium. Cell therapy also mitigated airway damage, reducing basement membrane irregularity and fragmentation, as well as disorganization and detachment of the airway epithelial cell layer (Table

3, Fig. 6). Y chromosome DNA was not detected in lung tissue at 5 weeks in cell-treated groups. TGF-β, PDGF, and IGF mRNA expressions in lung tissue were higher, while VEGF expression was lower in E-SAL compared to C-SAL. BMDMC administration led to an increase in IGF and VEGF mRNA expressions, and a reduction in TGF-β and PDGF expressions Obeticholic Acid price Fulvestrant clinical trial (Fig. 7). In the present murine model of pulmonary elatase-induced emphysema, early intravenous BMDMC therapy led to: (1) reduction in mean linear intercept, fraction area of alveolar collapse and hyperinflation; (2) reduction in

the number of mononuclear cells and neutrophils and collagen fiber deposition in lung tissue; (3) increase in elastic fibers in the alveolar septa; (4) decrease in airway epithelium and alveolar-capillary membrane damage as well as elastic fiber breakdown; (5) reduction in the degree of lung apoptotic cells and caspase-3 expression, and (6) improvement of right ventricular wall thickness and right ventricular many area followed by a reduction in collagen fibers in lung arterial vessels. Our findings suggest that the lung and heart may be protected by a mechanism linked to a balance between growth factors. In the present study, emphysema was induced by multiple intratracheal instillations of porcine pancreatic elastase. This model, initially described in NMRI

mice (Luthje et al., 2009), leads to lung, cardiovascular, and systemic impairment (Antunes and Rocco, 2011), and induced lung and cardiovascular damage in our C57BL/6 mice. Moreover, this method of emphysema induction yields lengthy progressive inflammatory and remodeling processes, in addition to alveolar destruction. The present study is the first to demonstrate the protective effects of early BMDMC administration to the lung and heart in experimental pulmonary elastase-induced emphysema. To identify this homing of bone marrow cells to the lung parenchyma, we used PCR of Y chromosome-specific sequences. However, Y chromosome DNA was not detected in lung tissue at day 28 in cell-treated groups, suggesting that the benefits we observed probably result from paracrine effects.

Various validated systems for testing the components of HPV E1 he

Various validated systems for testing the components of HPV E1 helicase and viral DNA replication using transient transfection of E1 and E2 expression plasmids or using purified enzymes in vitro have been reported ( Liu et al., 1995, Kuo et al., 1994 and Fradet-Turcotte et al., 2010). Further research is also needed in understanding the effects of CDV on the productive replicative cycle of low-risks HPVs and the organotypic epithelial raft cultures appear to be the ideal system to perform these investigations as they reproduce epithelial differentiation in an ex vivo system. A fully productive 3-dimensional tissue culture system

for production of high yields of infectious HPV-18 virions PF-06463922 mouse was first described in 2009, with multiple published applications since then (Wang et al., 2009). This system appears to be also more appropriate to analyse drug-metabolism because nucleoside metabolism in cell monolayer cultures (especially with immortalized and transformed cells)

are considerably abnormal compared to 3-dimensional tissues, where most cells are quiescent. Moreover, uptake of small molecules is substantially altered in rapidly dividing monolayer cells that do not have cell–cell junctions. Nucleotide synthetic pathways have exquisitely coordinated balancing of de novo production of the ribonucleoside and the deoxyribonucleoside INCB024360 chemical structure triphosphates, and these regulatory responses are also heavily influenced by salvage of nucleosides from broken down RNA and DNA or from the general circulation. Exogenous agents such as inhibitors of these synthetic or salvage pathways (eg. hydroxyurea, methotrexate) or from nucleoside analogues (eg. 5-FU) can substantially alter this balancing network. Whether CDV or other ANP’s

have an impact on the normal distribution of ribo- and deoxyribo-nucleosides and their phosphorylated derivatives should be investigated. How CDV and other ANPs impact ribonucleoside diphosphate reductase, the main source tuclazepam of deoxynucleotide synthesis in virally infected cells should be considered, as well as the consequences of cell growth in the presence of CDV with respect to ribosomal RNA transcription and processing. One of the major findings regarding CDV-antitumor activities points to the potential use of the drug in the therapy of non-viral induced tumors such as glioblastomas. Also, further research will be necessary to elucidate the effects of CDV in several signalling pathways compared to PME derivatives and other chemotherapeutics in order to highlight (dis)similarities and understand their mechanisms of action. We are grateful to the Geconcerteerde Onderzoeksacties (GOA), Krediet no. 10/014 and to the Program Financing (PF-10/08) of the KU Leuven for funding. “
“Integrase inhibitors (INIs) are an important addition to the HIV infection treatment armamentarium. Licensed in 2007, raltegravir (RAL, Merck Laboratories) is the first INI approved for clinical use (FDA, 2007).

01, p < 0 01, and p = 0 04, respectively) Percentage contributio

01, p < 0.01, and p = 0.04, respectively). Percentage contribution of left and right parts, respectively, was: 45.30 ± 9.10% and 54.33 ± 12.9%

in Vrc,a, 45.00 ± 6.52% and 55.00 ± 6.52% in Vab, and 48.04 ± 5.38% and 52 ± 5.31% in total chest wall volume (Vcw). A significant negative correlation (r = −0.878 and p < 0.01) was found between Borg Scale after the 6MWT and the Vrc,a (left side) 5-Fluoracil cost during ILB ( Fig. 2). A linear correlation at the limit of significance (r = 0.468 and p = 0.049) was present between Vrc,a (left side) and LV ejection fraction during ILB ( Fig. 3). No significant correlations were recorded between variations of Vrc,a (left side) during IMT and 6MWD (r = −0.064 and p = 0.79), LSVE (r = 0.03 and p = 0.89), and LVSD (r = −0.11 and p = 0.695). The present study demonstrates significant differences in regional distribution of thoracoabdominal volumes between patients with heart failure associated with cardiomegaly and healthy controls. More specifically, the left side of the lower

rib cage is characterized by lower displacement during ILB breathing. Regional distribution differences in selleck chest wall volume are correlated with other functional parameters, namely left ventricular ejection fraction and dyspnea. Patients with CHF were characterized by impaired lung function, as shown by the lower FVC, FEV1, and FEF values compared to healthy individuals. Some authors attribute these findings to respiratory muscle weakness, lung fluid imbalance, and exaggerated neurohumoral activity (Rutten et al., 2006, Johnson et al., 2000, Daganou et al., 1999 and Puri et al., 1994). Agostoni et al. (2000) proposed an influence of cardiomegaly on pulmonary function. According to this study, patients with cardiomegaly, defined by an increase Orotic acid in cardiothoracic index, showed lower FEV1 and FVC. In the present study, cardiomegaly was determined by the increase

in left ventricular systolic and diastolic diameters. This amplification in cardiac chambers could be considered a competing factor with pulmonary parenchyma, leading to deterioration in pulmonary function (Olson et al., 2006, Olson et al., 2007 and Agostoni et al., 2000). In relation to inspiratory muscle strength, MIP < 70% was used as an inclusion criterion for the CHF group. Respiratory muscle weakness and physical deconditioning may be involved in the increase in respiratory work during hyperpnea at the time of task performance (Witte and Clark, 2005 and Clark et al., 1995). Reduced functional capacity, assessed by the 6MWT, associated with less strength and endurance generated by inspiratory muscles are factors that worsen CHF patient prognosis and survival (Meyer et al., 2001). This study recorded a decrease in distance covered and a rise in the Borg index after the 6MWT for CHF group patients when compared to healthy subjects. During ILB, the CHF group displayed smaller volume variations in the lower rib cage compared to controls.

The great problem with coring for environmental and land-use cons

The great problem with coring for environmental and land-use construction has been its misuse for prospection for sites and assessment of site stratigraphy (e.g., McMichael et al., 2012, Rossetti et al., 2009 and Sanaiotti http://www.selleckchem.com/products/epacadostat-incb024360.html et al., 2002). Coring superficially with narrow-diameter manual augurs or drills is no way to discover archeological deposits because too little material is sampled and collected. Even at known archeological sites, such cores fail

to reflect the presence archeological deposits, not to speak of their stratigraphy. Mechanized drilling adds the problem of churning strata and mixing materials of different age. Dating has been inaccurate and inadequate in Amazonia. Materials in natural soil

and sediment strata are wrongly assumed to be the same age. Experimental research shows unequivocally that such strata combine materials of very different ages, because of bioturbation, translocation, geologic carbon, or human disturbance (Piperno and Becker, 1996, Sanaiotti et al., 2002, Roosevelt, 1997 and Roosevelt, 2005). Also, inattention to stratigraphic reversals in transported alluvium has resulted in anachronistic environmental reconstructions (e.g., Coltorti et al., 2012 and van der Hammen and Absy, 1994). Most natural strata in paleoecological investigations are not dated except by metric extrapolations from isolated radiocarbon dates (e.g., Bush et al., 1989), a problematic procedure because sedimentation rates Etoposide nmr in lakes and rivers always vary through time. Every interpretation zone needs to have multiple dates, for credible chronologies. Radiocarbon and stable carbon samples are rarely run on botanically identified unitary objects (e.g., Hammond et al., 2007), lessening tuclazepam dating precision and interpretive specificity. Most researchers misinterpret infinite radiocarbon assays (designated by laboratories with the symbol “>”) as radiocarbon dates (e.g., Athens and Ward, 1999 and Burbridge et al., 2004). But such results only mean

that the carbon was too old to radiocarbon date, and alternate dating techniques are necessary. Argon/argon dating of volcanic ash is rarely dated but can give very precise absolute ages. Optically stimulated luminescence (OSL) also can check radiocarbon dating but when used alone, it gives imprecise dates (Michab et al., 1998). For all these reasons, most Amazonian sequences lack verified chronologies, making it difficult to use them to understand environmental or cultural change. Firm chronology has emerged from direct dating of large samples of ecofacts and artifacts from recorded context with multiple techniques. Important potential sources of information are the biological materials preserved in archeological and agricultural sites and the sediments lakes, ponds, and rivers, which catch pollen, phytoliths, and charcoal (Piperno and Pearsall, 1998).