Materials and methods Patients and tissue specimens One hundred and fifty-three of colon cancers obtained between August 1999 and December 2003 were identified from our pathology files in NVP-BGJ398 supplier department of Pathology at the First Clinical Hospital of Shanxi Medical University, China. After review, 39 cases with synchronous other malignant tumors, familial adenomatous polyposis, colitis ulcerosa or Crohn’s disease, using neoadjuvant therapy,

lack of confirmatory surgical material, and/or clinical follow-up were excluded from this study. The remaining 114 cases were selected for SPARC, VEGF and CD34 staining. A pair of tissue samples for each case was collected from the tumor tissues and their corresponding non-diseased colon. The protocol of this study was approved by our Institutional Review HDAC inhibitor Board before all specimens were find more examined by the experienced pathologists. Histological examination was carried out on paraffin-embedded sections stained with hematoxylin

& eosin (H&E). The patients were followed-up in a range of 4-110 months (median = 53 months), the mean survival time was 99.0 months and the five-year survival rate was 76.0%, median survival time was 81.7 months. Seventy two of these patients were found to be recurrence or metastasis with the metastatic sites of lymph nodes, stomach, spleen, liver, pancreas, SPTLC1 ovary, cervix and bladder, and forty two cases died during the follow-up period. Other clinical and pathologic parameters were obtained from the pathological reports, including tumor differentiation, lymphocytic infiltration in the tumor interstitial and the TNM stage, and all of these data were reviewed and confirmed by the pathologists in our department (Table 1). Table 1 Clinicopathologic characteristics of the colon cancer patients Parameters No. of patients(%) Parameters No. of patients(%) Age (median, 59 years)   N2 13(11.4) < 59 48(42.1) Recurrence/distant

metastasis   ≥ 59 66(57.9) Yes 23(20.0) Gender   No 91(79.8) Men 54(47.4) L/infiltrationa   Women 60(52.6) Yes 41(36.0) Tumor size(average 5.0)   No 73(64.0) < 5.0 52 (45.6) depth of invasion   ≥ 5.0 62(54.4) T2 15(13.2) Localization   T3 88(77.2) colon ascendens 27(23.7) T4 11 (9.6) flexura hepatica 22(19.3) Distant metastasis   colon transversum 6(5.3) M0 102(89.5) flexura lienalis 8(7.0) M1 12 (10.5) colon descendens 6(5.3) TNM staging   colon sigmoideum 45 (39.5) I 11(9.6) Tumor differentiation   II 47(41.2) low 16(14.0) III 44(38.6) moderate 68(59.6) IV 12(10.5) high 30(26.3) Clinical outcome   Lymph node metastasis   Disease free 72(63.2) N0 65(57.0) Metastasis or recurrence 72(63.2) N1 36(31.6) Death 42(36.