For each lithology, we calculated the density of domestic wells (number of wells per km) in Monterey and Santa Barbara Counties (Table A1). These densities were used to estimate the number of wells in each section within SLO County. Additional explanation is provided in the Appendix. The 1990 US Census surveyed households for their “Source of Water” (census question code H023). Possible responses to the survey question

included “Public system or private company”, check details “Individual well”, or “Some other source”. Individual well included wells that were drilled or dug. The Source of Water question was dropped after the 1990 decadal census for unknown reasons and has not been surveyed since. The Summary Tape File 3 tabular data were downloaded from the

US Census website, along with the geographic boundaries of the 1990 census tracts (see http://www2.census.gov/census_1990/1990STF3.html). The tabular data were converted to Excel and then joined to their related census tract polygons in a GIS software package. In total, there were 5568 unique census tracts in the Excel table. When joined, Quizartinib purchase the total number of households using a domestic well was 464,272. Distributing the population using domestic supply evenly across a census tract would result in an over-generalized spatial homogeneity of domestic households, especially in the larger census tracts. Census tracts vary from <0.01 km2 to 20,697 km2. Instead, we used the estimated number of domestic wells in the PLSS sections within a census tract to distribute the number of households across the census tract. A census ratio (CR  c) was computed for each census tract: equation(4) CRc=DHcDWcwhere DHcDHc is the reported number of households using domestic-well water within a census tract and DWcDWc is the sum of the number

of domestic wells in the PLSS sections within a census tract. The census ratio was used to assign a number of households to each well within a census tract. In turn, the number of households within each section or other geographical boundaries can be computed (see Section 2.3). For census tracts Casein kinase 1 that contained households using domestic wells, but did not contain domestic wells according to the well-log survey, the density of households using domestic wells was assumed to be uniform across the census tract. Within the GAMA program, groundwater quality is evaluated on a basin scale, and not on a section scale (Belitz et al., 2003). Therefore, we aggregated section-scale estimates of the number of domestic wells and households dependent on groundwater into GUs in order to compare one unit to another. Groundwater Units do not follow exact PLSS section or census tract boundaries lines. Therefore, it was necessary to calculate domestic wells and census households in the sliver polygons formed when GUs intersect these irregular boundaries.