With increasing fungal concentration, the MST of the T rapae pop

With increasing fungal concentration, the MST of the T. rapae population decreased and the hazard ratios increased, indicating faster speed of kill by M. brunneum compared to B. bassiana ( Table 3). At the highest concentration (1 × 109 conidia ml−1) the MST was 4 days for M. brunneum and 6 days for B. bassiana. In the no-choice situation,

the treatment had no significant effect on the proportion of non-ovipositing females (binomial GLMM: likelihood ratio test (LRT) = 3.6306, df = 2, p = 0.1648). The number of eggs laid by T. AZD5363 mw rapae was found to be significantly dependent on the treatment (Poisson GLMM: LRT = 9.834, df = 2, p = 0.00732; Fig. 1). More eggs were laid in hosts in M. brunneum inoculated patches compared to the control

patches (Poisson GLMM: Z = −2.555, df = 1, p = 0.01063) and compared to host patches inoculated with B. bassiana (Poisson GLMM: Z = −2.755, df = 1, p = 0.00587). IPI-145 nmr The numbers of eggs found in D. radicum larvae did not differ for those in control and B. bassiana inoculated host patches (Poisson GLMM: Z = 0.213, df = 1, p = 0.832; Fig. 1). Females that later died from mycosis from either of the fungi laid significantly more eggs than non-mycosed females (Poisson GLMM: Z = 4.856, df = 1, p < 0.001), but no effect was found between number of eggs laid and female longevity (Poisson GLMM: Z = −0.886, df = 1, p = 0.3755). For M. brunneum treatments, the proportion of mycosed T. rapae was 0.81, and their mean (±SD) longevity post-experiment was 5.9 (±1.1) days (n = 13). The proportion of mycosed T. rapae due to B. bassiana was 0.56 (n = 9) and they showed a mean (±SD) longevity of 7.4 (±2.8) days. In the choice between fungal inoculated and non-inoculated host patches, T. rapae females did not discriminate between either M. brunneum and control (binomial GLMM: Z = 0.915, df = 1, p = 0.360), or B. bassiana and control (binomial GLMM: Z = 0.918, df = 1, p = 0.359). In the dual choice experiment, the Rho proportion

of mycosed T. rapae due to M. brunneum was 0.39 (n = 7), and their mean (±SD) longevity post-experiment was 9.1 (±2.9) days while for B. bassiana the proportion of mycosed T. rapae was 0.44 (n = 8) with longevity of 7.6 (±1.4) days. When offered a choice between healthy host larvae and M. brunneum infected ones, T. rapae laid significantly more eggs in the healthy larvae (binomial GLMM: Z = −3.283, df = 1, p = 0.00103; Fig. 2A). However the proportions of eggs laid in healthy host larvae and those infected by B. bassiana were not significantly different (binomial GLMM: Z = −1.321, df = 1, p = 0.187; Fig. 2B). No parasitoids succumbed to mycosis by M. brunneum and the majority (79%, n = 19) survived until 14 days post-experiment, while the proportion of mycosed T. rapae due to B. bassiana was 0.48 (n = 11) with a mean (±SD) longevity of 10.1 (±2.6) days. In this study D. radicum larvae were susceptible to both M. brunneum and B. bassiana. Compared to B.

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