This enzyme regulates the phosphatidylglycerol content via a phospholipase C-type degradation mechanism [24]. Another gene involved in lipid metabolisms, glycerophosphoryl diester phosphodiesterase (GT222042) was repressed during the infection. This enzyme has both phosphoric diester hydrolase and glycerophosphodiester phosphodiesterase activity and is involved in the metabolism of glycerol and lipids [25]. Protein synthesis and destination We identified several selleck products TDFs that were related to protein metabolism in our study. Among these were genes that encoded ribosomal proteins and enzymes involved in degradation. The expression of two ubiquitin-protein

ligases (GT222065 and GT222065) and one 50 S ribosomal protein L15 (GT222023) were repressed, whereas another 50 S ribosomal protein L15 (GT222024) was induced. This suggests that the infection results in a general induction of protein turnover, which could reflect an adaptive response in the plants to remove

misfolded proteins that have accumulated as https://www.selleckchem.com/products/Staurosporine.html a result of stress [23]. Signal transduction Three of the modulated genes had signal transduction and/or gene regulation functions. They corresponded two AZD1152 chemical structure transducin family protein (GT222030 and GT222029) that were repressed by infection and a serine/threonine protein kinases (GT222061) that was induced during infection. Serine/threonine protein kinases are a group of enzymes that catalyse the phosphorylation of serine or threonine residues in proteins,

with ATP or other nucleotides acting as phosphate donors. The phosphorylation of proteins on serine, threonine, or tyrosine residues is an important biochemical mechanism to regulate the activity of enzymes and is enough used in many cellular processes [26]. The two down-regulated proteins were identified as members of the transducin family and contained WD40 domain. This domain is found in several eukaryotic proteins that with wide variety of functions, which include adaptor/regulatory modules in signal transduction, together with proteins involved in pre-mRNA processing, and cytoskeleton assembly [27]. It is unclear how these changes contribute to the response of Mexican lime tree to infection. Conclusion We believe that this study is the first reported analysis of the expression of genes involved in the interaction of Mexican lime trees with “” Ca. Phytoplasma aurantifolia”". The cDNA-AFLP technique allowed several novel genes to be identified from Mexican lime trees, because a significant proportion of the TDFs are not currently represented in citrus databases. Our data showed that infection resulted in the down-regulation of Mexican lime tress transcripts in all major functional categories. However, certain genes that were required for plant-pathogen interactions were modulated positively during infection at the symptomatic stage.