The relative standard deviations were better than (14.44) for Zn, (2.75) for Ni, (0.17) for Pb, (0.049) for As, (0.032) for Cd, (0.082) for Co, (0.055) for U, (34.33) for Cu, and (7.57) for Cr.3. thenthereby Results and DiscussionH, O stable isotope ratios from water juice and C stable isotope ratios from pulp and the content of 9 elements (Cu, Cr, Ni, Zn, Pb, Co, As, Cd, and U) of 31 Romanian apple juices collected from four Transylvanian areas are presented and discussed in this study. The aim of this study was to measure the 2H/1H, 18O/16O, 13C/12C ratios of these juices and their elemental profile and to compare these results with those already reported in literature for apple single-strength juices in order to show the particularities of Transylvanian apple juices from the 2010 harvest.3.1.
Isotope Ratio Mass Spectrometry (IRMS) DataThe H, O, and C stable isotope composition of plant material is generally related to the climate conditions (relative humidity, temperature, amount of precipitation), geographical characteristics (distance from the sea or other evaporation source, height, latitude) of the area where the plant grow [13�C16], and the plant variety [3, 17].By comparison with commercial fruit juices, it is known that authentic juices have elevated ��18O and ��2H content of water as compared to water from rediluted products made using tap water which is relatively depleted in heavy oxygen and hydrogen isotopes [2].
Even if this method of detection of illegally adulteration of commercial fruit juices is a very powerful one, it requires a sufficient number of data for authentic juices coming from different geographical origin and from different years, especially since the isotopic parameters of fruit juices show remarkable variability depending on the climate factors [1]. Thus, the variation of ��18O values in water of authentic apple single-strength juice from central Europe (Germany, Italy, Belgium, Poland, Austria, Hungary, and Czechia) for the seasons 1991�C1994 obtained by Rossmann [1] showed a relatively large range of variation, between ?1��and ?5��with a mean value of about ?4��. The single-strength apple juices investigated by us shown a mean value of ��18O = ?4.2��for apples from Transylvania with small Anacetrapib differences among the mean values for the considered regions. The variation of the mean value of each region was between ?3.7��in Maramures region and ?4.5��in Cluj area, and the variation range was between ?2.68��(Maramures area) and ?5.27��(Cluj area) (Figure 1). Determinations of ��D parameter of water from apple juice have shown a mean value for deuterium ��D �� ?46.