The characteristics of the study groups as well as the blood ChE activities were reported previously (Vera et al., 2012). Briefly, PP and PR groups were similar in terms of demographical characteristics and habits.
Only 1.2% (RP) reported alcohol consumption (less than two alcoholic beverages/week) and 5% and 6.2% (RP and PP, respectively) had smoked during pregnancy. Comparing the average blood ChE activity of RP vs. PP, plasma BChE decreased significantly (20%, p < 0.01), suggesting maternal anticholinesterase pesticide exposure in PP. As shown in Table 1, placental ChE activity was affected by the sampling period. The average ChE activity of placental homogenates increased significantly Ruxolitinib mouse 76% (p < 0.001) in PP. A representative gel of placenta samplesfrom RP and PP groups is shown in Figure 4. The comparison of RP sample (line 1) and PP samples (lines 2 and 3) demonstrated a higher intense band in RP sample and suggest the same location of BChE plasma tetramer. As expected, in the present study the measured enzymatic activity in placenta homogenates was almost fully inhibited by eserinehemisulfate (Figure 1A). The results observed with this generic inhibitor of ChE, confirmed previous reports. Our results, are also in consonance with those ofFant and Harbison(Fant and Harbison, 1981) and Derewlany et al. (Derewlany et al., 1994) who previously showed activity on both
ChE from different subcellular fractions of placenta. Inhibitors incubations showed that one of Baf-A1 order them presents the properties of a vertebrate BChE: high sensitivity to serine (Figure 1A)and iso-OMPA (Figure 1 C). It must be noted that the incubation with the chemical iso-OMPA, specific inhibitor of BChE, resulted in significant but non complete inhibition. This result suggested that the remaining activity reflects the relative contribution
of AChE to ASCh hydrolysis. In fact, there was another which presents all the properties of Glycogen branching enzyme a vertebrate AChE: high sensitivity to eserine and BW284c51 (Figure 1B). Also, a partial sensitivity to BW284c51 was observed for AChE. As stated, enzymatic activity using ASCh represents combined AChE and BChE activities. Therefore, the substrates preference of placenta homogenate samples suggests that BChEcontributed almost to the 75% to the total ChE hydrolysis of ASCh (Figure 2).In accordance with the substrates preference assay, the non-denaturing gradient gel electrophoresis of placenta samples revealed only one band when stained with both AChE (Figure 3 A) and BChE (Figure 3B), showing that BChE activity represents total placental ChEs activity detected by this method. In agreement, the content of ChE mRNAs by RT-PCR in human kidney samples, showed that this organ possesses abundant BChE activity and less AChE activity in the form of GPI-anchored species (Muñoz-Delgado et al., 2010).