Similarly, the A1b strains, FRAN005, FRAN006, FRAN007, FRAN008, FRAN009, FRAN010, FRAN014, and FRAN015 all derive from cottontail rabbit from one state park in Illinois, with 5 or fewer SNP differences distinguishing these PCI-32765 supplier strains (Figure 3, Table 1). The A2 strains, FRAN001, FRAN027 and FRAN028, were considered likely derivatives of the avirulent strain 38 (Jellison); SNP based phylogenetic clustering confirms this assumption (Figure 3, Table 1). Within type B nodes, strains from Russia and North America were associated with node 64 Baf-A1 purchase (B2 strains), whereas only strains derived from North America (B1
strains) were associated with node 52 (Figure 3, Table 1). Overall, all unique type B strains (FRAN029, OR96 0246, OR96 0463, FRAN025, KY99 3387, CA99 3992, FRAN012, IN00 2758, KY00 1708 and MO01 1673) were resolved using whole genome SNP analysis. Table 3 summarizes the SNP content
for each of the major nodes identified in our phylogenetic analysis (Figure 2). The differentiating SNPs and maximum SNP separation numbers are indicators of the diversity within each node, as these represent SNP differences between members of the node (rather than SNP differences relative to the reference genome). The differentiating SNPs are the number of locations at www.selleckchem.com/products/VX-680(MK-0457).html which two or more member strains have differing base calls. Maximum SNP separation is the maximum number of SNP differences that are found between Dichloromethane dehalogenase any two members of the node. As expected, the SNP diversity is greatest within subspecies (type
A and type B) and decreases within clades; B1, A1a and A1b strains showed the least diversity (maximum SNP separation of 76, 75 and 38, respectively). Typing methods have previously revealed less diversity within type B than type A strains [2, 21–23]. Similarly, our data show less diversity among type B isolates, with a maximum SNP separation of 602 when the Japanese holarctica strain FRAN024 is excluded from this analysis (B*). However, when all type B isolates, including the Japanese holarctica strain FRAN024, are included in the analysis, our data indicates a similar level of diversity for types A and B (maximum SNP separation of 2779 and 2833, respectively). Table 3 SNP content of the major nodes identified in the phylogenetic tree (cladogram) Node Sub-species/clade/sub-clade Number of strains per node Total SNPs Total SNPs in LVS genome Total SNPs in SchuS4 unique sequence Common SNPs Unique SNPs Differentiating SNPs Maximum SNP separation 50 B 13 3771 3686 85 5 2837 3656 2833 51 B* 12 1154 1115 39 6 233 1060 602 52 B1 7 779 750 29 385 164 161 76 64 B2 5 705 677 28 7 153 628 549 4 A 26 8653 8559 94 2905 514 3765 2779 39 A2 6 6003 5919 84 3789 358 316 201 5 A1 20 7306 7291 15 4953 323 497 176 8 A1a 9 7001 6993 8 5491 277 129 75 23 A1b 10 7030 7022 8 5537 234 71 38 * contains all the type B strains with the exception of FRAN024, Japanese holarctica strain.