Sera were analyzed by Enzyme Linked Immunosorbent Assay (ELISA) for evidence of anti-MUC1 antibody. We found marked overexpression of MUC1 in IBD, most of which was hypoglycosylated. On colon specimens from healthy age-matched controls, we found low levels of luminal MUC1 and no alteration in its glycosylation. We detected antibody to MUC1 in sera of IBD patients as well as controls, and in a limited number of IBD samples examined longitudinally,

we could correlate the rise and fall of antibody levels with clinical disease severity. MUC1 is overexpressed and hypoglycosylated in pediatric IBD and may play an important role in the pathogenesis of IBD, and thus warrants further study as a potential therapeutic target. Similarly, antibodies to MUC1 may influence IBD and should be explored as potential diagnostic or prognostic markers.”
“IntroductionBloodstream PI3K inhibitor infections (BSIs) and central line infections

remain among the major causes of morbidity and mortality in transplant recipients because of prolonged neutropenia and mucosal damage. The objective of this study was to determine the frequency and outcome of bacterial and fungal isolates from patients undergoing allogeneic hematopoietic stem cell transplant.

Materials and methodsThis study was conducted at the Aga Khan University and Hospital’s bone marrow transplant unit. All patients who underwent an allogeneic stem cell transplant with matched sibling/parent donor were included. The study period ranged from April R788 order 2004 to December 2012. Transplantation was performed according to institutional protocols. All patients were admitted in single rooms selleck kinase inhibitor with positive pressure

and high-efficiency particulate air filters. Ciprofloxacin, fluconazole, and valaciclovir were used for standard prophylaxis, which was started at the time of conditioning. All blood cultures were obtained at clinical suspicion of systemic infection, mainly documented as fever (temperature of >38.5 degrees C). BSIs and line infections were defined as isolation of bacterial or fungal pathogen from at least one blood/central line culture.

ResultsIn total, 101 of 108 patients developed febrile neutropenia. In the 101 patients, 245 documented febrile episodes occurred. There were 40 culture-positive episodes and 205 culture-negative episodes. Of these 40 culture-positive episodes, 22 patients had bloodstream isolates and 18 had central line isolates. The medianstandard deviation time of febrile neutropenia was day 7 +/- 2days (range: day -3 to day +13). The most common bloodstream isolate was Escherichia coli (n=9) followed by Staphylococcus epidermidis (n=5). One patient developed Fusarium infection. In central line infections, S.epidermidis was the most common organism (n=8). In 2 patients with central venous catheters, Candida albicans was the isolate. Transplant-related mortality from sepsis occurred in 9.2%.

ConclusionE.