Our information showed that all 6 phenolic acids tested possess a dose dependent and time dependent inhibitory antiproliferative effect on T47D cells. Nonetheless, a dif ferential impact for each phenolic acid was located, with IC50 values various in the nanomolar for the picomolar range. The time course of phenolic acids varies equally. Without a doubt, caffeic acid exerts its action later on than other phenolic acids, suggesting a different mode of action. Ferulic acid, structurally relevant to caffeic acid, shows a bimodal impact, with a brief time part as well as a lengthy time component. Last but not least, all other phenolic acids present a half maximal effect, attained just after 2 days. Evaluating the structures of the dif ferent phenolic acids, presented in Fig.
1, our information propose the two hydroxyl groups on the phenolic ring along with the three carbon side chains are the two essential to the antiproliferative exercise. The shortening from the selelck kinase inhibitor side chain creates a loss with the antiproliferative exercise, that’s far more obvious in each the methylation of 1 or each OH group and the p OH substitution located in syringic acid and sinapic acid. Additionally, shortening on the side chain confers an enhanced IC50 worth, indicating a attainable increase on the transmembrane transit from the compounds or, alternatively, an greater interaction with an unknown membrane constituent. To examine the doable mode of action of basic phenolic acids on T47D cell development, we now have concentrated on two from the 6 phenolic acids, caffeic acid, which is by far the most potent, and PAA, that’s a precursor for your synthe sis of other much more complicated molecules.
Furthermore, we’ve got examined reduced concentrations of those phenolic acids comparable with these identified during the entire body immediately after con sumption of meals. In contrast to polyphenols, we have now not detected any interaction of those two com pounds with both estrogen, progesterone or adrenergic receptors in T47D cells. Cell cycle analysis exposed that phenolic acids selleck chemical improve the amount of nonapoptotic cells while in the S phase and lessen the G2 M phase from the cell cycle. Additionally, each substances induced apoptosis inside five days. PAA appears to become a additional potent inducer of apoptosis than caffeic acid. Phenolic acids were reported to possess a significant antioxi dant action in different programs. We have now therefore investigated irrespective of whether the antiproliferative and pro apop totic results of caffeic acid and PAA had been correlated with their inhibition of H2O2 toxicity, an indirect measurement of their antioxidant exercise. A differential impact amongst caffeic acid and PAA was located. Without a doubt, caffeic acid didn’t show any notable shift of your H2O2 induced toxicity.