J Infect Dis 2003, 187:1424–1432.PubMedCrossRef 9. Enright M,
Spratt G: A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. RAAS inhibitor Microbiol 1998, 144:3049–3060.CrossRef 10. Sá-Leão R, Pinto F, Aguiar S, Nunes S, Carriço JA, Frazão N, Gonçalves-Sousa N, Melo-Cristino J, de Lencastre H, Ramirez M: Invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines revealing heterogeneous behavior of clones expressing the same serotype. J Clin Microbiol 2011, 49:1369–1375.PubMedCrossRef 11. Hanage WP, Kaijalainen TH, Syrjanen RK, Auranen K, Leinonen M, Makela PH, Spratt BG: Invasiveness of serotypes and clones of Streptococcus pneumoniae among children in Finland. Infect Immun 2005, 73:431–435.PubMedCrossRef 12. Sandgren A, Sjostrom K, Olsson-Liljequist B, Christensson B, Samuelsson A, Kronvall G, Henriques Normark B: Effect of clonal and serotype-specific properties on the invasive capacity of Streptococcus pneumoniae. J Infect Dis 2004, 189:785–796.PubMedCrossRef 13. Sjostrom K, Spindler selleck chemical C, Ortqvist A, Kalin M, Sandgren A, Kuhlmann-Berenzon S, Henriques-Normark B: Clonal and capsular types decide whether pneumococci will act as a primary or opportunistic pathogen.
Clin Infect Dis 2006, 42:451–459.PubMedCrossRef 14. Jefferies JM, Smith AJ, Edwards GFS, McMenamin J, Mitchell TJ, Clarke SC: Temporal analysis of invasive pneumococcal clones from Scotland illustrates fluctuations in diversity of serotype and genotype in the absence of pneumococcal conjugate
vaccine. J Clin Microbiol 2010, 48:87–96.PubMedCrossRef 15. Elberse KEM, van de Pol I, Witteveen S, van der Heide HGJ, Schot CS, van Dijk A, van der Ende A, Schouls LM: Population structure of invasive Streptococcus pneumoniae in the Netherlands in the pre-vaccination era assessed by MLVA and capsular sequence typing. Plos One 2011, 6:1–11. 16. Lefevre JC, Faucon G, Sicard AM, Gasc AM: DNA fingerprinting of Streptococcus pneumoniae Oxalosuccinic acid strains by pulsed-field gel electrophoresis. J Clin Microbiol 1993, 31:2724–2728.PubMed 17. Hall LM, Whiley RA, Duke B, George RC, Efstratiou A: Genetic relatedness within and between serotypes of Streptococcus pneumoniae from the United Kingdom: analysis of multilocus enzyme electrophoresis, pulsed-field gel electrophoresis, and antimicrobial resistance patterns. J Clin Microbiol 1996, 34:853–859.PubMed 18. Aanensen DM, Spratt BG: The multilocus sequence typing MAPK inhibitor network: mlst.net. Nuc Acids Res 2005, 33:W728-W733.CrossRef 19. Koeck J, Underwood A, Brunetaud J, Leroy P, Granger-Ferbos A, Koeck J, Pichon B: Multiple-Locus variable-number tandem-repeat analysis of Streptococcus pneumoniae and comparison with MLST.