J Bacteriol 2003,185(2):1027–1036.PubMedCrossRef 36. D’Argenio DA, Calfee MW, Rainey PB, Pesci EC: Autolysis buy Epacadostat and autoaggregation in Pseudomonas aeruginosa colony morphology mutants. J Bacteriol 2002,184(23):6481–6489.PubMedCrossRef 37. Allesen-Holm M, Barken KB, Yang L, Klausen M, Webb JS, Kjelleberg S, Molin S, Givskov M, Tolker-Nielsen T: A characterization of DNA release
in Pseudomonas aeruginosa cultures and biofilms. Mol Microbiol 2006,59(4):1114–1128.PubMedCrossRef 38. Shrout JD, Chopp DL, Just CL, Hentzer M, Givskov M, Parsek MR: The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional. Mol Microbiol 2006,62(5):1264–1277.PubMedCrossRef 39. Rahme LG, Stevens EJ, Wolfort SF, Shao J, Tompkins RG, Ausubel FM: Common virulence factors for bacterial pathogenicity in plants and animals. Science 1995,268(5219):1899–1902.PubMedCrossRef 40. GDC-0994 Holloway BW, Krishnapillai V, Morgan AF: Chromosomal genetics of Pseudomonas
MI-503 molecular weight . Microbiol Rev 1979,43(1):73–102.PubMed 41. Wilder CN, Diggle SP, Schuster M: Cooperation and cheating in Pseudomonas aeruginosa : the roles of the las , rhl and pqs quorum-sensing systems. ISME J 2011,5(8):1332–1343.PubMedCrossRef 42. Liberati NT, Urbach JM, Miyata S, Lee DG, Drenkard E, Wu G, Villanueva J, Wei T, Ausubel FM: An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci USA 2006,103(8):2833–2838.PubMedCrossRef 43. Simon R, UPAP : A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria. Nat Biotech 1983, 1:784–791.CrossRef 44. Becher A, Schweizer HP: Integration-proficient Pseudomonas aeruginosa vectors for isolation of single-copy chromosomal lacZ and lux gene fusions. Biotechniques 2000,29(5):948–950–952.PubMed 45. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP: A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located
DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Resveratrol Gene 1998,212(1):77–86.PubMedCrossRef 46. Heeb S, Blumer C, Haas D: Regulatory RNA as mediator in GacA/RsmA-dependent global control of exoproduct formation in Pseudomonas fluorescens CHA0. J Bacteriol 2002,184(4):1046–1056.PubMedCrossRef 47. Schweizer HP: Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 1991,97(1):109–121.PubMedCrossRef 48. Horton RM, Cai ZL, Ho SN, Pease LR: Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 1990,8(5):528–535.PubMed 49. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248–254.PubMedCrossRef 50.