: DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 2005,434(7035):864–70.PubMedCrossRef 22.

Gorgoulis VG, Vassiliou LV, Karakaidos P, Zacharatos P, Kotsinas A, Liloglou T, Venere M, Ditullio RA Jr, Kastrinakis NG, Levy B, Kletsas D, Yoneta A, Herlyn M, Kittas C, Halazonetis TD: Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature 2005,434(7035):907–13.PubMedCrossRef 23. Bartkova J, Bakkenist CJ, Rajpert-De Meyts E, Skakkebaek NE, Sehested M, Lukas J, Kastan MB, Bartek J: ATM activation in normal human tissues and testicular cancer. Cell Cycle 2005,4(6):838–45. Epub 2005 Jun 13PubMedCrossRef MK-8931 in vitro 24. Pusapati RajuV, Robert J, et al.: ATM promotes apoptosis and suppresses tumorigenesis in response to Myc. Proc Natl Acad Sci USA 2006,103(5):1446–1451.PubMedCrossRef 25. Haidar MohammadA, Kantarjian Hagop, Manshouri Taghi, et al.: ATM Gene Deletion in Patients with Adult Acute Lymphoblastic Leukemia. CANCER 2000, 5:1057–1062.CrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions JZ participated in the design of the study and performed the statistical analysis. LL carried out cell culture and flow cytometry assay, participated in the animal experiment. YZ participated in irradiation for cells

and animals. SL conceived of the find more study, and participated in its design and coordination and helped to draft the manuscript. JF designed the study, BCKDHA performed the rest of the experiments and wrote the manuscript. All authors read and approved the final manuscript.”
“Introduction The exact chemical composition of FWGE, which is currently used as nutriment for

cancer patients is not completely known [1]. It contains two quinones, 2-methoxy benzoquinone and 2,6-dimethoxybenzquinone that likely play a significant role in exerting several of its biological properties [2]. Preclinical in vitro and in vivo data suggested antiproliferative, antimetastatic and immunological effects of FWGE [1–7]. In cell lines studies, FWGE induced programmed cell death via the caspase – PARP-pathway [7, 8]. But the exact mechanism by which this multi-molecule composition triggers cell death is still obscure. In previous studies several groups could demonstrate that FWGE interferes with enzymes of the anaerobic CP673451 order glycolisis and pentose cycle [2, 9, 10]. Known targets are the transketolase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase and hexokinase which are necessary for the allocation of precursors for DNA-synthesis [9]. Also involved in DNA-synthesis is ribonucleotide reductase [6]. This enzyme is upregulated in various types of cancer and is an attractive target in cancer chemotherapy.