CB 33. REGULATION OF CERULOPLASMIN BY HYALURONAN IN GLIOMA PROGENITORS S. Tye, A. G. Gilg, J. Knapp, L. Olson, J. R. Bethard, C. A. Welsh, Z. Rumbolt, I. Takacs, B. P. Toole, and B. L. Maria, Charles P. Darby Childrens Analysis Institute, Healthcare University of South Carolina, Charleston, SC, USA The multicopper oxidase enzyme Ceruloplasmin was not too long ago shown to be secreted by a desmoplastic infantile ganglioglioma that probably arises from multipotent purchase Roscovitine progenitor cells. The purpose of this review was to determine whether Ceruloplasmin is regulated by hyaluro nan, a big polysaccharide that promotes anti apoptosis, invasion, and drug resistance in malignant cells. By means of FACS evaluation, we isolated a side population of cells in the rat C6 glioma cell line that expressed ABCG2 and have been highly drug resistant by virtue of their BCRP efflux of chemotherapy, on top of that, we isolated BCRP constructive neurospheres in the U87 human glioma cell line.
C6SP cells cul tured on matrigel for 10 days expressed the two neuronal and glial markers. PCI-34051 Western blotting showed that C6SP cells and U87 neurospheres contained considerably a lot more Ceruloplasmin than their respective mother or father lines. Antagonizing hyaluronan/CD44 interactions by treating C6SP and U87 neurospheres with hyaluronan oligomers decreased Ceruloplasmin manufacturing. IL 1 beta improved Ceruloplasmin and HIF 1 alpha manufacturing in C6 cells over in IL six, and IL six improved Ceru loplasmin and HIF 1 alpha production much more in C6SP. C6 and C6SP cells engrafted into the rodent central nervous procedure both expressed abundant Ceruloplasmin. Taken with each other, these final results propose that glioma cells and their progenitor subpopulations express Ceruloplasmin in vitro and in vivo, Ceruloplasmin manufacturing in glioma progenitors is heavily depen dent on hyaluronan/CD44 interactions, and downstream from hyaluro nan/CD44 interactions, inflammatory mediators modulate Ceruloplasmin production differently in glioma progenitors.
Ongoing scientific studies will deter mine how hyaluronan mediated Ceruloplasmin manufacturing contributes to anti apoptosis, invasion, and drug resistance in glioma progenitor cells. CB 34. CYTOSTATIC Results OF ISOTYPE SELECTIVE AKT INHIBITOR CANDIDATES IN

MODEL PEDIATRIC BRAIN TUMORS Timothy Van Meter, Anil Kumar, Catherine Dumur, William C. Broaddus, and Gary Tye, Departments of Neurosurgery, Anatomy and Neurobiology, and Pathology, School of Medicine, Virginia Commonwealth University Health Systems, VA, USA Previous studies from our laboratories reported characterization of AKT isotype expression and activity in PNET and medulloblastoma cell lines an enhanced sensitivity to cisplatin induced cell death in the presence of micromolar doses of PH domain directed AKT inhibitors. We deter mined the growth suppressive results of AKT inhibitors that have been characterized for their isotype selectivity.