CAR−/− mice demonstrated NASH inspite of decreased bodyweight/adiposity. Thus, nuclear receptors
PXR and CAR interfere with energy metabolism and apparently play an important protective role in Ar-induced liver injury. Disclosures: The following people have nothing to disclose: Banrida Wahlang, Keith C. Falk-ner, Ming Song, Heather B. Clair, Russell A. Prough, Matthew C. Cave Aim; Nonalcoholic fatty liver disease (NAFL) morbidity rate in Western countries is close to 20–30%. Nonalcoholic fatty liver disease (NAFLD) can progress to nonalcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma. In spite of its high prevalence, up till now here is no proven effective treatment for NAFLD. Along with the “obesity epidemic,” the worldwide prevalence of NAFLD is increasing selleckchem rapidly Palbociclib concentration and is generally assumed to be a consequence of obesity-induced insulin resistance. On the other hand, not all obese individuals are insulin resistant, nor are all insulin-resistant individuals obese. MicroRNAs (miRs) are a class of
small non-coding RNAs that function to control gene expression. Although miRs play a key role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD) and diabetes mellitus (DM), detailed mechanisms of this pathogenesis remains unclear. We found that miR-27b increased in liver biopsy specimens of NAFLD patients with DM using microarray analysis, as compared with controls. The aim of this study was to investigate whether overexpression of miR-27b in liver could cause fatty liver formation and insulin resistance, and to examine mechanism of NAFLD and DM onset in murine model. METHODS; Five-week-old male C57BL/6J mice were randomized into 2 groups(n=16 mice): basal diet (BD)-fed control mimic (BD-Con, n=4), BD-fed miR-27b-mimic (BD-miR-27b, n=4).
In this study, miR-27b mimics is injected intravenously at 7mg/kg. We comfirmed the target genes of miR-27b using quantitative RT-PCR analysis. insulin serum concentrations were measured Galactosylceramidase by a local laboratory for clinical examinations. As an alternative method for assessing insulin resistance (IR), the homeostasis model assessment of IR (HOMA-IR) was calculated using the following formula: fasting insulin (mU/mL) plasma glucose (mg/dL) / 405. Result; BD-miR-27b significantly showed steatosis using oil red o staining and increased hepatic tryglyceride content, as compared BD-Con. In the analysis of fat accumulation related gene expression, hepatic Peroxisome proliferator-activated receptor α (PPARα) and Microsomal triglyceride transfer protein (MTTP) are significantly decreased. At the same time, BD-miR-27b showed hyperinsulinemia and insulin resistance. In the analysis of insulin resistance related gene expression, hepatic Insulin receptor substrate 1 (IRS-1) is significantly decreased.