Antibacterial activity was assayed by measuring the diameter of the zone of inhibition formed around the well using standard (Hi-Media) scale. The experiment done in triplicate and the average values were calculated for antibacterial activity. Minimum inhibitory concentration (MIC) was defined as the lowest concentration where no visible turbidity was observed in the test tubes. The concentrations were determined by the method described

by Vollekova11 with minor modification was employed. The MIC was determined for the micro organisms that showed maximum sensitivity XAV-939 nmr to the test extracts. In this method the broth dilution technique was used, where the leaf extract was prepared to the highest concentration of 25 mg/ml (stock concentration). By adding sterile distilled water serially diluted (two fold dilutions) using the nutrient broth and it is later inoculated

with 0.2 ml standardized suspension of the test organisms. After 18 h of incubation at 37 °C, the test tubes were observed for turbidity. The lowest concentration of the tube that did not show any visible growth can be considered as the minimum inhibitory concentration. selleck screening library It is estimated that total ash value in leaves is 10.83%, acid insoluble ash and water soluble ash shows the value 4.66% and 3.16% respectively. The extractive value of methanol is more followed by aqueous, chloroform and petroleum ether with 20.12%, 6.98%, 4.36% and 2.14% respectively. Phytochemical screening of crude extracts of the aerial part of the T. angustifolia reveals the presence of alkaloids, tannin, steroids, phenol, saponins, flavonoids in aqueous and methanolic extracts where as carbohydrates, tannins, oils and fats were present in Petroleum ether and chloroform extract. In addition to this chloroform extract also contains flavonoids and phenols. The

antimicrobial activity of different extracts against the test organisms with varying zones of inhibition ranging from 09 to 20 mm (Fig. 1) has revealed the antimicrobial potency of this plant. Methanolic extract showed highest zone of inhibition against E. coli (20 mm) followed by P aeruginosa, S typhimurium, E aerogenes and K pneumonia. The aqueous extract PDK4 showed greater potential against E. coli > E. aerogenes > P. aeruginosa > K. pneumonia > S. typhimurium. Chloroform extract shows moderate inhibitory effect on these organisms. The result of MIC assay is shown in Table 1 Methanol extract of T. angustifolia exhibited the highest antibacterial efficacy against E. coli at 0.78 mg/ml and least efficacy was shown by chloroform against S. typhimurium, P. aeruginosa and E. coli which was inhibited at 12.5 mg/ml concentration. Plants are important source of potentially bioactive constituents for the development of new chemotherapeutic agents. It has been well documented that the antimicrobial compound are abundantly present in medicinal plants.