Three independent experiments done in triplicate were realized. Statistical analysis Data are

expressed as mean +/- standard deviation (SD). Statistical analysis was performed with Student’s t test. A p value < 0.05 was considered statistically different. Nucleotide sequence accession number The DNA sequence reported in this paper has been deposited in GenBank under accession number JF699754. Acknowledgements This study was supported by the Institut National de la Recherche Agronomique (INRA) and the Ministère de l'Education Nationale de la Recherche et de la Technologie (MENRT). We thank N. Rouhier for his technical advices and his technical supports. We thank S. Payot-Lacroix and M. Genay-Bernard for critical reading of the manuscript. References 1. Kosikoski FV, Mistry VV: Volume 1: Origins and Principles. 1997. in Cheese selleck inhibitor and Fermented Milk Foods, r.e. Westport, Editor. 2. Wouters JA, Rombouts FM, de Vos WM, Kuipers OP, Abee T: Cold shock proteins and low-temperature response of Streptococcus thermophilus CNRZ302. Appl Environ Microbiol 1999,65(10):4436–42.PubMed 3. Perrin C, Guimont C, Bracquart P, Gaillard

JL: Expression of a new cold shock protein of 21.5 kDa and of the major cold shock protein by Streptococcus thermophilus after cold shock. Curr Microbiol 1999,39(6):342–0347.PubMedCrossRef 4. Varcamonti M, Arsenijevic S, Martirani L, Fusco D, Naclerio G, De Felice M: Expression of the heat shock gene clpL buy JQ1 of Streptococcus thermophilus is induced by both heat and cold shock. Microb Cell Fact 2006, 5:6.PubMedCrossRef 5. Martirani L, Raniello R, Naclerio G, Ricca E, De Felice M: Identification of the DNA-binding protein, HrcA, of Streptococcus thermophilus. FEMS Microbiol Lett 2001,198(2):177–82.PubMedCrossRef 6. Derre I, Rapoport G, Msadek T: CtsR, a novel regulator of stress and heat shock response,

controls clp and molecular ROS1 chaperone gene expression in gram-positive bacteria. Mol Microbiol 1999,31(1):117–31.PubMedCrossRef 7. Kilstrup M, Jacobsen S, Hammer K, Vogensen FK: Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactis . Appl Environ Microbiol 1997,63(5):1826–37.PubMed 8. Zotta T, Asterinou K, Rossano R, Ricciardi A, Varcamonti M, Parente E: Effect of inactivation of stress response regulators on the growth and Linsitinib manufacturer survival of Streptococcus thermophilus Sfi39. Int J Food Microbiol 2009,129(3):211–20.PubMedCrossRef 9. Fleuchot B, Gitton C, Guillot A, Vidic J, Nicolas P, Besset C, Fontaine L, Hols P, Leblond-Bourget N, Monnet V, Gardan R: Rgg proteins associated with internalized small hydrophobic peptides: a new quorum-sensing mechanism in streptococci . Mol Microbiol 2011,80(4):1102–19.PubMedCrossRef 10. Neely MN, Lyon WR, Runft DL, Caparon M: Role of RopB in growth phase expression of the SpeB cysteine protease of Streptococcus pyogenes . J Bacteriol 2003,185(17):5166–74.PubMedCrossRef 11.